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Abstract

Psychrotrophic bacteria are able to grow at 7 C or lower but had higher growth temperature called psychrotrophs. They are commonly found in different cold environments such as deep
oceans, Antarctic regions, seasonally cold environments as their potential ability to sustain high temperature. Psychrotrophs produce cold active enzymes which reveals higher catalytic activities
as compared to mesic and thermic conditions.Most psychrotrophs produce hydrolytic heat stable extracellular enzymes like proteases, lipases, phospholipases etc in milk or dairy products stored
at low temperature. In this present study cooled buffalo milk was used for the isolation of psychrotrophic pseudomonas species and its protease production was studied on different pH and
temperatures. On skim milk agar a zone of hydrolysis showed proteolytic activity of
Pseudomonas and it is measured by selection ratio.Selection ratio(SR) = diameter of zone of
hydrolysis / diameter of colony in mm. proteolytic activity was optimized on different pH and temperatures. Results showed that maximum protease production was observed at 37 C and pH

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