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Abstract
Fruit rot disease of Pomegranate ( Punica granatum L). is caused by Alternaria alternata. During present investigation Aa-21 resistant isolate of this pathogen was used. Present
investigation was undertaken to find out the antimicrobial potential of Pseudomonas fluorescensin vitro. Four environmental conditions were testes with different concentration and
ranges , exhibited significant mycelial growth inhibition of A. alternata. Physical factors such as
H
0
temperature and P influence the activity of biocontrol agent , At 25 C Percent inhibition was
(84.42 %) , at P
H
5.0 ( 84.77%) , at 30gm carbon source percent inhibition was ( 82.85%), and at
3gm nitrogen source it was (80.18%). Decrease in concentration of carbon and nitrogen source there is decrease in growth of resistant isolate; carbon and nitrogen have been established as
essential elements in the infection process of plant pathogens.