Main Article Content
Abstract
In the present study the variety of cluster bean namely Ankur Rani was used to induce
genetic variability. For these study two chemical mutagens, namely EMS of different
concentration such as 0.05%,0.10%,0.15% and SA of different concentration such as 0.01%,
0.02% and 0.03% were used. The seeds of variety Ankur Rani of Cluster bean were obtained
from Local market of Aurangabad (Maharashtra).These seeds were subjected to mutagenesis by
using chemical mutagens. M1 generation was raised and effect of these mutagens on different
parameters
like
germination
percentage,
seedling
height,
leaf
morphological changes,
chlorophyll chimeras, pollen sterility, and plant survival percentage. Both the mutagens had
shown an inhibitory effect on seed germination. The 0.15% EMS showed highest germination
percentage. The 0.03% SA showed lowest germination percentage. Seedling height was found to
increase as concentration of both the mutagens. But exceptionally it is found to be