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Abstract
Stem rot disease caused by Sclerotium rolfsii Sacc. is one of the most important disease of groundnut causing major crop losses. To pursuit for the effective Bacillus spp.as microbiological control of Sclerotium rolfsii Sacc., 189Bacillus spp. were isolated from different rhizospheric
nichesof healthy plants, and primarily screenedin vitrofor the antagonistic activity against Sclerotium rolfsii, by dual culture technique. During the primary screening, Bacillus spp. RRR15,found highly effective in killing the phytopathogen, Sclerotium rolfsii in Vitro.
TheBacillus spp.RRR15, effectively slaughter the growth of phytopathogen, Sclerotium rolfsii whose percent inhibition recorded as 87.5. The Bacillus spp. RRR15 later identified as Bacillus
amyloliquefaciens RRR15 by 16 S rRNA sequencing.