Main Article Content
Abstract
Optimization of fermentation conditions for improved production of L-glutaminase by Pseudomonas aeruginosa MM-2 was carried out. Effect of different physicochemical parameters
namely
concentration, carbon and nitrogen sources were studied. The isolated marine bacteria Pseudomonas aeruginosa MM2 showed the maximum production of enzyme after 48 hours of
incubation
period,
initial
pH,
temperature,
Nacl concentration, L-glutamine
incubation, at pH 8, temperature 35°C, 3% Nacl concentration, 1.5% L-glutamine concentration. The glucose as carbon source and beef extract as nitrogen source has supported the maximum yield of L-glutaminase. After optimization of different fermentation parameters the yield of L-