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Abstract
Irradiated pollen can be used to arouse gynogenetic haploid production. The dose of radiation is one of important factor for controlling haploid production. In order to know whether gamma irradiated pollen is viable or non-viable or they have germination capacity or not, experiment on effect of gamma radiation on marigold pollen viability and germination percentage was conducted. Pollen of marigold cultivar ‘Pusa Narangi Gainda’ was irradiated with 0, 50,100, 150, 200, 250, 300 Gy gamma radiation in Gamma Radiation Chamber 5000, ICAR-IIHR, Bengaluru. Pollen viability percentage of these pollens was recorded by staining with Alexander stain and aceto carmine separately. Pollen germination test was done using hanging drop method. Maximum pollen viability of 98.67% and 99.33% was reported when control pollen (which was not gamma irradiated) was stained with Alexander stain and aceto carmine stain respectively.