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Abstract
Two representative genera of rod shaped, gram-positive bacteria of class Bacilli includs few species of Lactobacillus and Bacillus can produce bacteriocin which is a polypeptide complex and shows antimicrobial activity. Bacteriocin is a class of antimicrobial and/or antibiotics which inhibit the biosynthesis of cell membrane of few of the gram negative bacteria including E.coli. But the strength of antimicrobial activity is very low and produced in very low concentration which can be improved by various genetic manipulation techniques such as mutation by UV radiations and different optimized conditions which were found favorable for increasing the bacteriocin concentration like pH 6-9, temperature 360C, selective MRS media, incubation time varying from 0-96 hours and varying glucose concentration ranging from 1-5%.