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Abstract
Probiotic when administrated in adequate amount confers health benefits to the host. Literatures have suggested the beneficial effects of probiotics on lifestyle disorders viz. hypercholesterolemia, diabetes and obesity in both humans and animals. Probiotics must survive the harsh conditions of the gastrointestinal tract and colonize, even temporarily, in the colon to confer health benefits. Therefore, in the present study probiotic attributes of Lactobacillus amylovorus MTCC8129 and L. bifermentum MTCC3818 were evaluated in vitro. Different properties viz. acid tolerance, BSH activity, cell surface hydrophobicity, auto-aggregation and antimicrobial activity were studied. Both strains have shown the good growth at pH 2.0 and 3.0 when compared to growth at pH 6.5. However, L. bifermentum MTCC3818 exhibited high acid tolerance compared to L. amylovorus MTCC8129.