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Nigella sativa L. belongs to the family Ranunculaceae is a popular plant in indigeneous medicine all over the world used for treating various diseases. The present study was undertaken to analyze the phytochemical components and for screening the antimicrobial activity of seed extracts of Nigella sativa Linn. The extract is prepared using various solvents such as water, ethanol, and chloroform and physicochemical characters of Nigella sativa seed extract was carried out by standard procedures. The preliminary phytochemical screening of the extract showed the presence of alkaloid, flavanoids, phytosterol, saponins, steroids, tannins and terpenoids in ethanol extract. For this, the antibacterial properties of Nigella sativa L. seed extract were tested against gram positive (staphylococcus aureus) and gram negative(Escherichia coli) bacteria and the antifungal activity of Nigella sativa L. seed extract were tested against Candida albicans, andAspergillusnigerby using different solvents namely chloroform, ethanol, and water. The ethanol seed extract of Nigella sativa L. showed significant antibacterial activity against Escherichia coli and antifungal activity against Candida albicans. Thus we can scientifically conclude that the black cumin seeds extract could be seen as an progressively valuable reservoir of bioactive compounds of substantial medicinal merit.
Key words: Physicochemical characters, Nigella sativa, antibacterial, antifungal